Recently, Lowry and Post-doctoral Researcher Astrid Avellan have made a breakthrough discovery: gold nanoparticles actually dissolve in freshwater environments, when they come into contact with mircroorganisms found on aquatic plants. During the process, gold ions are released, which will behave differently from the nanoparticles and could be toxic to some microorganisms.

The study did not measure toxicity so this doesn’t mean gold nanoparticles are harmful—instead, by better understanding their behavior in biologically active environments, scientists can ultimately use this knowledge to design better nanomaterials.

Their findings weree published in Nature Nanotechnology ("Gold nanoparticle biodissolution by a freshwater macrophyte and its associated microbiome").

“This study has opened our eyes to the importance of plants and the plant microbiome in determining the fate of engineered nanomaterials in freshwater environments,” said Lowry. “These plants, and biofilms in general, are important sinks for nanomaterials and are a fascinating compartment to study.”

The team looked at exactly what causes this transformation and how quickly it occurs. They conducted their tests in what is called a mesocosm—a controlled natural freshwater environment. The mesocosm, housed at the Center for Environmental Implications of NanoTechnology at Duke University, contains soil, sediment, water, plants, insects, fish, and microorganisms that ordinarily live in these natural environments.

Avellan and the research team released gold nanoparticles into the mesocosm water in very low amounts every week to mimic long-term, low dose inputs expected from nanomaterial uses. They wanted to see how the nanoparticles would behave in a complex, biologically active ecosystem.

After six months they found that 70% of the gold was accumulating with the aquatic plants, and that all of the gold nanoparticles had dissolved and changed to other forms of gold. When they took a closer look at the biofilm, or a sticky substance made up of bacteria and microorganisms found on plants, they found that the microorganisms released cyanide that was interacting with the gold nanoparticles. The gold nanoparticles dissolved (or ionized) and formed gold-cyanide along with other gold complexes that remained with the plants.

Microscopy image showing the microorganisms present in the biofilm found on subaquatic plants that cause the gold to dissolve.

Nanoparticles are aggregates of atoms forming particles between one and one hundred nanometers, or one-hundredth to one-thousandth of the thickness of a human hair. Their size confers novel properties that benefit many applications: they could treat water better, they could kill bacteria on a wound, they could create stronger but lighter materials.

“We found that gold was accumulating like crazy in the aquatic plants, which was not what we were expecting,” said Astrid Avellan. “So we dug into that and found that gold was associated with these plants, but it wasn’t nanoparticulate anymore.”

This is a major breakthrough because gold nanoparticles were thought to be a stable material, and have often been used as a tracer to understand how nanomaterials behave—if you find the nanoparticles then you know where the nanoparticles accumulate. The findings from this paper imply that even relatively inert metal nanoparticles like gold can actually dissolve when they interact with biofilm in water environments.

Video

CEE Professor Greg Lowry and Postdoc Astrid Avellan describe their work looking at how nanomaterials behave in very complex environments using mesocosms that represent freshwater, emergent wetlands. They studied the behavior of gold nanoparticles in the mesocosms and discovered that gold was not inert, as they expected, but rather was transformed quite a bit by organisms in the environment.

“The interactions of nanomaterials with the phytobiome can potentially be leveraged to benefit agriculture,” said Lowry. “The research community is only beginning to understand the role of the phtyobiome on plant productivity. This study indicates the potential to design nanomaterials that work together with the phytobiome to improve plant productivity. Successful interventions in agriculture will need to consider how to work synergistically with nature.”

Though the effects of the gold transformation need to be studied more, it is possible that it could be toxic to some organisms. The ions could also move faster and farther away than the nanoparticles, distributing differently in organisms and in the environment. The good news is that now researchers have discovered how and why they dissolve, so we can be smart about future uses and applications of nanoparticles—even leveraging this phenomenon for our benefit.

“Now we know why and in what conditions gold nanoparticles dissolve,” said Avellan. “So we can take this knowledge and use it to ouradvantage to design better materials.

Carnegie Mellon University. Posted: Aug 15, 2018.

Assuntos Conexos:
New testing finds synergistic combination leads to toxicity in nanomaterials.

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